Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.894
Helicobacter Pylori Regulates the Autophagy and Apoptosis Of CACO-2 Cells Through the Nrf2 Signaling Pathway
Abstract
To study the mechanism of Helicobacter pylori regulating autophagy and apoptosis of gastric epithelial cells through NF-κB signaling pathway. Helicobacter pylori (H. pylori, Hp) and human gastric epithelial CACO-2 cell lines were cultured in vitro. Hp treatment 0 h, 3 h, 6 h, 9 h and control group, 18 μmol·L-1NF-κB specific inhibitors were collected The CACO-2 cells treated with SN50 for 6 h and 18 μmol·L-1SN50 + Hp for 6 h were used to detect the autophagy rate and apoptosis rate of each group of cells by flow cytometry; the CACO-2 cells after Hp gradient time treatment were collected to extract each The total protein was detected by Western blot (Western Blot) to detect the expression of autophagy-related proteins Beclin1related X protein (Bax) and Nrf2. The autophagy rate and apoptosis rate of CACO-2 cells gradually increased with the extension of Hp action time, and there was a statistically significant difference between each time point and 0 h (P <0.01). The expressions of autophagy-related proteins Beclin1, apoptosis-related proteins Bax and Nrf2 gradually increased with the extension of Hp action time. Compared with 0 h, the difference in protein expression at each detection time point was statistically significant (P<0.01). Compared with the control group, the autophagy rate and apoptosis rate of CACO-2 cells were significantly enhanced after Hp treatment; the autophagy rate and apoptosis rate% of CACO-2 cells were significantly reduced after treatment with Sn50; after the combined treatment with Hp and Sn50, the autophagy of CACO-2 cells The rate and apoptosis rate were significantly higher than the control group, but lower than the Hp alone treatment group, the difference was statistically significant. Hp stimulation significantly promotes autophagy and apoptosis of CACO-2 cells. The mechanism may be related to the activation of NF-κB pathway.
Keywords
Helicobacter pylori; epithelial cells; autophagy; apoptosis; Nrf2