Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.900
Estradiol on Brain Pathological Changes in Ovariectomized Rats with Chronic Cerebral Ischemia
Abstract
To investigate the protective effect of Ligustrazine on osteoporosis in diabetic rats. 28 healthy female SD rats, weighing 350 g-420 g, were provided by a medical college. Twenty-eight female rats were randomly divided into four groups: normal control group (group A), sham ovariectomized ischemia group (group B), ovariectomized ischemia group (Group C) and estradiol benzoate treatment group (Group D), with 7 rats in each group. Estradiol benzoate was added 1 week after ovariectomy. After 3 weeks, the forebrain ischemia model was established by 2VO except the normal control group, and the experiment was continued for 5 weeks. The density of capillaries and capillaries of the model rats were observed by immunohistochemical staining, and the density of capillaries and capillaries of the model rats were observed. Finally, the data were statistically analyzed. Compared with the normal control group, the density of neurons and capillaries in ischemic group was significantly decreased. Compared with OVX + EB + 2VO group and shamovx + 2VO group, the density of neurons and capillaries in OVX + 2VO group was significantly lower than that in OVX + EB + 2VO group (P < 0.05). Compared with the normal control group, the density of hippocampal CA1 neurons in ischemic group was decreased. At the beginning of the experiment, there was no significant difference in the latency of each group. At the end of the experiment, except for the normal control group, the latency of other groups was significantly longer than that at the beginning of the experiment. The mortality of shamovx + 2VO group was 0.3, that of OVX + EB + 2VO group was 0.6, and that of OVX + 2VO group was 0.367. The mortality of OVX + 2VO group was higher than that of sham OVX + 2VO group and OVX + EB + 2VO group, but the difference was not statistically significant (P > 0.05). Estrogen has protective effect on the behavior changes of rats with cerebral ischemia before ovariectomy, and has beneficial effect on pathological changes of cerebral ischemia injury. Estradiol has a certain protective effect on chronic cerebral ischemia.
Keywords
Chronic Cerebral Ischemia, Ovariectomized Rats, Estradiol Research, Pathological Changes