Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.808
Effects of lncRNA-LET targeting miR-93-5p on proliferation and apoptosis of human dermal fibroblasts after ultraviolet radiation
Abstract
Objective: To explore the effects of circular ribonucleic acid G-protein subunit beta 1 (circGNB1) on the migration, invasion and apoptosis of colon cancer cells and its molecular mechanism.
Objective: To evaluate the effects of long non-coding RNA (lncRNA)-low expression in tumor (lncRNA-LET) on the proliferation and apoptosis of human dermal fibroblasts (HDFs) after ultraviolet (UV) radiation and relevant molecular mechanism.
Methods: Primary HDFs were radiated with UV ray (5 J·cm-2), and the expression levels of lncRNA-LET and micro RNA (miR)-93-5p were measured through fluorescence real-time quantitative polymerase chain reaction (RT-qPCR). HDFs were separately transfected with small interfering (si)-LET, si-negative control (NC), miR-93-5p, miR-NC, anti-miR-NC + si-LET, and anti-miR-93-5p + si-LET and after UV radiation. The cell proliferation viability, cell apoptosis rate, and expressions of Cyclin D1 and cleaved-caspase-3 were detected by cell counting kit-8 assay, flow cytometry and Western blotting, respectively. The dual luciferase reporter assay and RT-qPCR were conducted to verify the targeted regulatory relationship of lncRNA-LET with miR-93-5p.
Results: The expression level of lncRNA-LET in HDFs after UV radiation rose, while that of miR93-5p declined (P<0.05). After interference of lncRNA-LET expression or miR-93-5p overexpression, the HDFs after UV radiation exhibited increased proliferation rate and Cyclin D1 expression, and decreased apoptosis rate and cleaved-caspase-3 expression (P<0.05). On the contrary, the HDFs after UV radiation displayed lowered proliferation rate and Cyclin D1 expression, and raised apoptosis rate and cleaved-caspase-3 expression after suppressing both miR-93-5p and lncRNA-LET (P<0.05). The lncRNA-LET targeted miR-93-5p and negatively regulated the expression of miR-93-5p.
Conclusion: Interference of lncRNA-LET can facilitate the proliferation and repress the apoptosis of HDFs after UV radiation by targeting and regulating miR-93-5p expression.
Keywords
lncRNA-LET, miR-93-5p, UV, dermal fibroblast, cell proliferation, apoptosis.