Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1118
A 28-DAYS SUB-ACUTE TOXICITY STUDY IN SWISS ALBINO MICE TO ASSESS NEUROTOL PLUS (GLYCEROL AND MANNITOL IN COMBINATION) TOXICITY PROFILE
Abstract
Osmotic drugs continue to be the utmost communal treatment option accessible to control intracranial pressure (ICP). The combination of glycerol and mannitol is a better alternative and is currently available as the best therapy used to increase ICP. The purpose of this analysis was to investigate the effect of repetitive doses (28 days) of the combined neurotol plus, 20% mannitol and 10% glycerol on the safety profile. A 28-days sub-acute toxicity analysis was performed at 3 various level of dose, 5 ml / kg, 10 ml / kg and 20 ml / kg. Into 4 groups of 6 animals each; mice were divided randomly. As a final evaluation point, physical and biochemical parameters as well as hematological parameters related to liver toxicity and nephrotoxicity were examined. We also perform histopathological examinations to assess the toxicity of individual organs. This study showed no or minimal changes (at high doses) in several hematological, biochemical and physical parameters between the control group and the neurotol plus group. Taken together, the statistics from this analysis show that the amalgamation of glycerol and mannitol is not related with any grave side effects and is a safe beneficial option to reduce ICP
Keywords
neurotol plus; mannitol; glycerol; repeated dose toxicity; intracranial pressure