Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1123
The expression and clinical significance of serum miR-424, miR-205 and miR-155 in neonates with asphyxia
Abstract
Objectives: To detect the expression levels of miR-424, miR-205 and miR-155 in the serum of newborns with asphyxia, so as to provide scientific basis for the early diagnosis and treatment of the above mirnas in the serum in neonatal asphyxia.
Methods: The expression levels of miR-424, miR-205 and miR-155 in serum of 110 newborns with asphyxia were detected by RT-QPCR and analyzed statistically. The correlation between miRNA and the severity of asphyxia was analyzed.
Results: There were no statistically significant differences in gender, gestational age, birth quality, etiology, maternal age, twins, cesarean section and amniotic fluid abnormalities between mild asphyxia group (survival) and severe asphyxia group (death) (P > 0.05). There was statistical difference in the scores of Apagar for 1min and 5min between the two groups (P < 0.05), while there was no statistical difference in the scores of apagar for 10min (P > 0.05). All the 3 miRNA could be detected in the serum of asphyxiated newborns, and compared with the control group, the expressions of miR-424 and miR-155 were up-regulated and the expressions of MiR-205 were down-regulated, with statistically significant differences.
Conclusions: the expression of miR-424 and miR-155 in the serum of children with asphyxia is up-regulated, which is positively correlated with the severity of the disease, while the expression of miR-205 is down-regulated, which is negatively correlated with the severity of the disease. Therefore, detection of miRNA expression in the serum of newborns with asphyxia has very important clinical value for early diagnosis and prognosis judgment of newborn asphyxia.
Keywords
suffocation; The miRNA expression; Clinical significance; serum