Volume 29, Issue 3
DOI: 10.24205/03276716.2020.921
Dihydroartemisinin Inhibits Proliferation and Migration of Cervical Cancer Cells Depending on Mir- 214/PTEN Signal Axis
Abstract
Objective: This study was designed to probe into the inhibition of dihydroartemisinin on proliferation and migration of cervical cancer (CC) cells by miR-214/PTEN signal axis. Methods: Sixty-nine CC patients who were admitted in our hospital and 78 healthy people in the same period were selected as the research objects for prospective analysis. CC patients were included into a research group (RG), and healthy people were enrolled to a control group (CG). Human CC cells were purchased for biological analysis. The miR-214, PTEN expression and their predictive value in CC, and the relationship between the two were detected. The effects of miR-214 and PTEN on CC cells, and the changes of miR-214, PTEN and biological behavior in those cells after dihydroartemisinin treatment were assessed.
Results: The miR-214 and PTEN levels in CC patients in the RG were lower than those in the CG (p<0.05), and both of them had good predictive value. There was a positive correlation between miR-214 and PTEN. The migration rate of the miR-214 group was higher than that of the miR-214 group and the miR-NC group (p<0.05), and the apoptosis rate was lower (p<0.05). The PTEN and Bax expression in the miR-214 group was lower than that in the other two groups (p<0.05), and Bcl-2 was higher (p<0.05). The proliferation and migration rate of the PTEN-inhibition group were higher than those of the PTEN group and the NC group (p<0.05), and the apoptosis rate was lower (p<0.05). The PTEN and Bax expression in the miR-214 group was lower than that in other two groups (p<0.05), and Bcl-2 was higher (p<0.05). The miR-214 and PTEN expression in CC cells increased after dihydroartemisinin treatment (p<0.05), while cell proliferation and mobility decreased (p<0.05).
Conclusion: Dihydroartemisinin depends on miR-214/PTEN signal axis, which can inhibit the proliferation and migration of CC cells and has great application prospect in future diagnosis and treatment.
Keywords
dihydroartemisinin, miR-214/PTEN, cervical cancer