Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1001
Bioinformatics Analysis of MiRNA in Peripheral Blood of Patients with Severe Traumatic Brain Injury
Abstract
Objective: To analyze the target genes and functions of miRNA in peripheral blood of patients with severe traumatic brain injury (sTBI) by bioinformatics.
Methods: Gene microarray data from peripheral blood of sTBI patients and control group were retrieved from the GEO database. The differentially expressed miRNA were screened by bioinformatics method. The target genes were predicted, the biological functions and signal pathways were analyzed, and the miRNA and target gene regulatory networks were constructed.
Results: The microarray GSE21854 was obtained.145 differentially expressed miRNA were screened out and a total of 580 target genes were predicted. The functions of these target genes mainly included negative regulation of cell proliferation and negative regulation of transforming growth factor - β receptor signal pathway, which were mainly distributed in Ras signal pathway and transforming growth factor - β signal pathway, etc. The regulatory network map of miRNA and target genes showed that hsa-miR-125a-5p, hsa-miR-760, hsa-miR-217, hsa-miR-199a-3p and hsa-miR-543 were the core of regulation.
Conclusion: There are differentially expressed miRNA in peripheral blood of patients with sTBI. Hsa-miR-125a-5p, hsa-miR-760, hsa-miR-217, hsa-miR-199a-3p, and hsa-miR-543 are closely related to the progression of sTBI.
Keywords
Severe traumatic brain injury; MiRNA; Peripheral blood; Bioinformatics