Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1104
Baicalein Inhibits Migration of Breast Cancer Via Inflammatory and Apoptosis Cell Signaling Pathway
Abstract
Background: Breast carcinoma is one of the leading carcinomas in women in developed countries. Breast carcinoma is manifestation of different biological structures with distinct physiological features and clinical consequences. Baicalein, a form of flavonoid, isolated from Radical Scutellariae exerts specific pharmacological properties along with antioxidant nature. The current study evaluated the anti-mammary effect of baicalein on 7, 12-Dimethylbenz (α) anthracene (DMBA) induced breast cancer and explore the possible mechanism.
Methods: A single dose of DMBA (80 mg/kg) was injected in the mammary gland in the female rats to induce tumor. The female rats were divided into five groups- Group I-Normal control group; Group II- DMBA control (80mg/kg of BW); Group III- DMBA treated rats received baicalein 5 mg/kg of BW; Group IV- DMBA treated rats received baicalein 10 mg/kg of BW; Group V- DMBA-treated rats received baicalein 20 mg/kg of BW. At the end of experiment, tumor burden, incidence and volume were studied. Together, the markers of mammary tumors such as carcinoembryonic antigen (CEA), Lipid-bound Sialic Acid (LSA), Alpha-fetoprotein (AFP), Serum Total Sialic Acid (TSA), and Cancer Antigen 15–3 (CA 15–3) were estimated. Also, the pro-inflammatory cytokine, inflammatory mediators and apoptosis proteins were also studied.
Results: Compared to the DMBA control group, dose-dependent treatment with baicalein (P<0.001) significantly decreased the levels of AFP, CEA, LSA, TSA, and CA 15–3. Baicalein significantly (P<0.001) down-regulated the hepatic parameters such as alanine transaminase (ALT), aspartate transaminase (AST) , pro-inflammatory cytokines viz., interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6); inflammatory mediators such as nuclear factor kappa-B (NF-kB). Compared to DMBA control group rats, Baicalein significantly (P<0.001) reduced Bcl-2 expression and increased Bax and caspase-3 expression.Conclusion: Altogether, the above finding shows that the preventive effect of baicalin on DMBA induced mammary gland in Wistar rats.
Keywords
Breast cancer; DMBA; Baicalein; Inflammation; Apoptosis