Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.813
Correlations of LINC00858 and miR-363-3p Expressions in The Serum with Clinicopathological Characteristics of Liver Cancer Patients
Abstract
Objective: To investigate the correlations of the expressions of long non-coding ribonucleic acid (lncRNA) long intergenic non‑protein coding RNA 858 (LINC00858) and micro RNA (miR)-363-3p in the serum with the clinicopathological characteristics and prognosis of liver cancer patients. Methods: A total of 90 liver cancer patients admitted to and treated in our hospital from January 2017 to March 2019 were selected as liver cancer group, and 70 healthy people receiving physical examination in the same period were enrolled into control group. The expression levels of serum LINC00858 and miR-363-3p were detected using qRT-PCR. Then the liver cancer patients were assigned into LINC00858 high expression group (n=50), LINC00858 low expression group (n=40), miR-363-3p high expression group (n=30) and miR-363-3p low expression group (n=60) based on the average values of LINC00858 and miR-363-3p expression levels, and the correlations of LINC00858 and miR-363-3p expression levels with the clinicopathological characteristics of liver cancer patients were observed. The correlation between LINC00858 and miR-363-3p in the serum of liver cancer patients was analyzed by Pearson’s method. The receiver operating characteristic curves were plotted to analyze the diagnostic value of LINC00858 and miR-363-3p in liver cancer. All the patients were followed up for 2 years, and their 2-year survival was analyzed by Kaplan-Meier method.
Results: The expression level of serum LINC00858 was increased significantly (P<0.05), while that of miR-363-3p was decreased significantly (P<0.05) in liver cancer group compared with those in control group. LINC00858 was negatively correlated with miR-363-3p (r=-0.3946, P=0.0001), and the expression levels of LINC00858 and miR-363-3p had close relations to tumor thrombus, lymph node metastasis, tumor-node-metastasis (TNM) stage, differentiation degree and liver cirrhosis (P<0.05). As for LINC00858, the sensitivity was 65.56%, the specificity was 65.71%, the area under curve (AUC) was 0.673, the 95% confidence interval (CI) was 0.595-0.745, and the cutoff value was
1.18. The sensitivity, specificity, AUC, 95%CI and cutoff value of miR-363-3p were 83.33%, 37.14%, 0.597, 0.516-0.674 and 1.14, respectively. The survival rate of patients in LINC00858 high expression group was significantly lower than that in LINC00858 low expression group (P<0.05), but it was significantly higher in miR-363-3p high expression group than that in miR-363-3p low expression group (P<0.05).
Conclusion: Liver cancer patients have a high expression of LINC00858 and a low expression of miR-363-3p in the serum, LINC00858 and miR-363-3p are negatively correlated with each other, and they have close correlations with the TNM stage, tumor thrombus, differentiation degree, lymph node metastasis and other clinicopathological characteristics of patients, so they are conducive to the diagnosis of liver cancer and the evaluation of patient's prognosis.
Keywords
liver cancer, LINC00858, miR-363-3p, clinicopathological characteristic, prognosis.