Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1119
Induction of IRG-1 Reduces Reactive Oxygen Species Production by Bioactive Compounds of Anise (Pimpinella Anisum L.) on LPS-Activated Macrophages
Abstract
Plant extracts have become increasingly useful anti-inflammatory medicine and their contribution to primary healthcare in a significant population in the world. The research seeks to demonstrate the process of extracting plant’s bioactive constituents from Pimpinella Anisum L. and to show the role played by plant’s anise extract in Reactive Oxygen Species (ROS) production as reduced by expression of IRG1 by activated macrophages. The study outlines a new extraction method of Pimpinella Anisum L. at a low temperature (0 ℃). The bioactive components of the anise extract were detected using GC/MS analysis. The macrophages (RAW264.7) were stimulated by 5 μg/ml of lipopolysaccharides (LPS) from E. coli for 8 hours. Then, post-treatment of the anise extract was performed on the macrophages by 3 separate varied concentrations of 100, 250 and 400 μg/ml of Pimpinella Anisum L. extract for 24 hours. The aqueous plant extract showed high amounts of fifteen bioactive components bearing anti-inflammatory activities by GC/MS analysis. Further, the study provides a significant reduction in the cell viability were treated with the anise extract in comparison to the untreated LPS-activated macrophages. Moreover, mRNA levels and protein expressions of IRG1 gene were increased significantly resulted in ROS production being inhibited in post-treated LPS-activated macrophages. This is the first study reveals the impact of all chemical compositions in the Pimpinella Anisum L. extract’s essential oil indicated to have the required properties of an anti-inflammation and antioxidant effects that might reduce pathological inflammatory responses as a valid pharmacological agent
Keywords
Anise; Pimpinella Anisum L.; Essential oil; Phenolics; Antioxidant activity