Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1120
mmu-miR-146a-5p and mmu-miR-122-5 via targeting the multiple target genes played an important role in the Huangqi treatment of diabetes mellitus
Abstract
Background To explore the molecular mechanism of exercise and Huangqi on the effects of diabetes mellitus were examined for miRNA sequencing.
Mehtods Mouses in the model control group were fed with normal saline, mouses in the Huangqi group were intragastrically fed with huangqi for every day for 6 weeks, mouses in the Swimming group were trained with swimming per three days for for 6 weeks, and mouses in the Combine treatment groups were intragastrically fed with huangqi for every day for 6 weeks and trained with swimming per three days. We profiled the miRNA expression of skeletal muscle and myocardial tissues of mouse in those groups. Differentially expressed miRNAs were screened, and the databases, such as miRanda, miRDB, Targetscan and miRTarBase, were used to predict the target genes of differentially expressed miRNAs. Function alanalysis of the target genes of differentially expressed miRNAs was performed using Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) database.
Results In this study, mmu-miR-193b-3p, mmu-miR-5115, and mmu-miR-146a-5p were significantly differential expression in among groups in the skeletal muscle tissue. In the myocardial tissue, those miRNAs, such as mmu-miR-122-5p, mmu-miR-146a-5p and mmu-miR-215-5p were significantly differential expression in among groups. The miRNA-target genes interaction network was constructed to further investigate interaction correlations.
Conclusion The results implied that mmu-miR-146a-5p and mmu-miR-122-5 played an important role in the treatment of diabetes mellitus, via targeting the multiple target genes.
Keywords
diabetes mellitus; miRNA sequencing; exercise, Huangqi