Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1129
Changes of Serum Endothelial Microparticles in Mice with Septic Lung Injury
Abstract
Sepsis is an inflammatory response syndrome that causes the failure of multiple organs in the body. During the onset of sepsis, lung tissue is most vulnerable to damage due to its own physiological characteristics, thus forming an acute lung injury disease. As a carrier of inflammatory mediators, endothelial microparticles participate in endothelial function damage and may play an important role in acute lung injury. The purpose of this article is to explore the changes and mechanisms of serum endothelial microparticles in mice with septic lung injury. With 50 healthy mice as experimental subjects, the mice were divided into a septic lung injury observation group and a corresponding control group. Mice underwent cecal ligation and perforation to create an animal model of septic lung injury. Twelve hours after the model was successfully created, blood was drawn from the lung tissue of the mouse to detect the concentration of pro-inflammatory factor IL-1β, interleukin IL-6 and tumor necrosis factor TNF in the mouse serum, and detect the content of endothelial cell particles in the mouse serum, and use observe the ultrastructure changes of mouse lung tissue under microscope. The results of the study showed that compared with the control group, the serum pro-inflammatory factor IL-1β concentration level in the septic lung injury observation group increased by 18.4%, and the interleukin IL-6 concentration level increased by 21.5%. The level of tumor necrosis factor TNF increased by 24.8%. The content of endothelial microparticles in the serum of the observation group was (862.3±54.5)/μL, which was significantly higher than that of the control group (378.2±40.6)/μL. At the same time, the ultrastructure of the lung tissue of the observation group was destroyed.
Keywords
Septic Lung Injury, Mouse Serum, Endothelial Microparticles, Animal Model