Volume 29, Issue 5, 2020


DOI: 10.24205/03276716.2020.1130

Effect of rapamycin combined with nedaplatin in the treatment of bladder cancer and its influence on angiogenesis and invasion


Abstract
Objective To investigate the efficacy of rapamycin combined with nedaplatin in the treatment of bladder cancer and its influence on angiogenesis and invasion. Methods 96 cases of bladder cancer patients in our hospital and Shanghai Fudan clinical pathological diagnosis center from January 2015 to December 2019 were retrospectively analyzed. They were divided into two groups according to different preoperative chemotherapy methods, 48 cases in each group. The observation group was given rapamycin combined with nedaplatin chemotherapy, while the control group was only given nedaplatin chemotherapy. After 2 cycles of continuous chemotherapy, extensive hysterectomy and pelvic lymph node dissection were implemented. The clinical efficacy, surgical conditions, microvessel density (MVD), vascular endothelial growth factor (VEGF), type 1, 3a, 8 and β - Catenin in pathological and healthy tissues were compared between the two groups. Result After treatment, the total remission rate of the observation group was 77.08% (37/48), which was significantly higher than 52.08% (25/48) of the control group (P < 0.05); there was no significant difference in the operation time and intraoperative blood loss between the two groups (P>0.05). The rates of vascular invasion, parauterine invasion and lymph node metastasis in the observation group were 2.08% (1/48), 4.17% (2/48), 4.17% (2/48), which were significantly lower than 14.58% (7/48) of the control group). MVD and VEGF in the lesions of the two groups were significantly higher than those in the healthy tissues (P < 0.05), but the MVD and VEGF in the lesion tissues of the observation group were significantly lower than those in the control group, and the Pearson correlation analysis showed that there was a positive correlation between MVD and VEGF (P<0.05); Wnt1, Wnt3a, wnt8 and β - Catenin in in the lesion tissues of the two groups were significantly higher than those in the healthy group However, the levels of Wnt1, Wnt3a, wnt8 and β - Catenin in the lesion tissues of the observation group were significantly lower than those of the control group (P < 0.05); there were no grade III to IV liver and kidney injury in the two groups during the treatment, and there was no significant difference in the incidence of bone marrow suppression and gastrointestinal reaction between the two groups (P>0.05). Conclusion rapamycin combined with nedaplatin has a significant effect in bladder cancer patients, which can effectively regulate tumor angiogenesis and cancer cell invasion, and improve the disease remission rate, with less side effects and high safety

Keywords
bladder cancer; rapamycin; nedaplatin; angiogenesis; invasiveness

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