Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1147
Therapeutic effect of Astragalus Chinese herbal compound on children with thalassemia and its influence on KLF1 gene expression
Abstract
Objective: To observe the effect of Astragalus Chinese herbal compound on children with thalassemia and its influence on KLF1 gene expression.
Methods: Using a randomized controlled double-blind clinical study, 108 patients with β-thalassemia were randomly assigned into the traditional Chinese medicine treatment group and the placebo control group. The children in the traditional Chinese medicine treatment group were given Astragalus Chinese herbal compound, and the control group was given placebo granules 12 weeks. Observe blood routine and fetal hemoglobin ratio and other hematological indicators. And real-time fluorescent quantitative PCR reaction was used to detect the mRNA level of KLF1 gene.
Results: After the 12-week course of treatment, the Hb value of the children in the Chinese medicine group was 82.25±6.10 g·L-1 and the control group was 66.20±11.50 g·L-1. The difference between the two groups was statistically significant (P<0.04). The proportion of HbF after treatment in the Chinese medicine group was also significantly higher than before treatment (61.08%±13.02% vs. 52.31%±17.16%, P <0.01). Real-time fluorescent quantitative PCR experiment results showed that traditional Chinese medicine treatment had a significant effect on the expression of KLF1 gene. After treatment, the expression level of KLF1 gene was significantly lower than before treatment (0.33±0.19 vs. 0.81±0.40, P <0.01). TCM syndromes in the treatment group The scores were lower than those of the control group (P<0.05).
Conclusion: Astragalus Chinese herbal compound has a good effect on the treatment of β-thalassemia in children, and its molecular mechanism may be related to the decreased expression of KLF1 gene.
Keywords
β-thalassemia; Astragalus Chinese medicine compound; Hb; KLF1