Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1166
Inhibition of miR-223-5p attenuates proliferation and invasion of papillary thyroid carcinoma cells by targeting TIMP3
Abstract
Background: Papillary thyroid carcinoma (PTC), as an endocrine tumor of young and middle-aged, has increased significantly incidence rate in the past ten years. The ability of invasion and metastasis is significantly related to the prognosis of the disease. High throughput sequencing results showed that miR-223-5p expression was significantly up-regulated in PTC tissues than that in normal thyroid tissues. The aim of this study was to investigate the effect of miR-223-5p on the proliferation and invasion of PTC cells, and to explore its potential molecular mechanism.
Methods: The PTC tissues and adjacent normal tissues of 6 patients with PTC were collected during thyroidectomy. PTC cell lines of KTC-1, K1 and BCPAP was used as in vitre models. RT-PCR and western blot analysis were used to detect the miR-223-5p in protein and mRNA level. MTT assay was conducted to study the the proliferation of PTC cells. Transwell assay was used to detect the invasion of PTC cells.
Results: The miR-223-5p expression was upregulated in PTC tissues and PTC cells. Inhibition of miR-223-5p attenuated the proliferation and invasion of PTC cells. In terms of mechanism, we found that tissue inhibitor of metalloproteinase-3 (TIMP3) is a target gene of miR-223-5p. TIMP3 could be negatively regulated by miR-223-5p. Finally, miR-223-5p silencing suppresses the proliferation and invasion of PTC cells via regulating TIMP3.
Conclusion: Our results demonstrated that miR-223-5p inhibits the proliferation and invasion of PTC cells by targeting TIMP3, which indicating that miR-223-5p may be a new target for the treatment of PTC in the future and provides a new perspective for the PTC diagnosis.
Keywords
Papillary thyroid carcinoma; miR-223-5p; TIMP3