Volume 29, Issue 5, 2020
DOI: 10.24205/03276716.2020.1167
Effect of Amniotic Membrane Transplantation on Limbal Stem Cells in Alkaline Burn Rats
Abstract
The cornea is located on the outer layer of the eyeball and is very vulnerable to injury. After corneal alkali burn, the epithelial layer of the cornea is seriously damaged due to alkaline saponification. Limbal stem cells, as the regeneration source of corneal epithelial cells, can quickly migrate to the center to cover the wound surface and form new epithelium. This article studies the effect of amniotic membrane transplantation on stem cells of the limbs of alkaline burn rats, which provides strong support for the treatment of corneal diseases. In this paper, ACH was used to encapsulate limbal stem cells to be transplanted to the site of alkali burn of the rat cornea to provide necessary seed cells for the repair of alkali burn of the cornea. To observe its effect on wound repair, it is urgent to provide effective tissue engineering treatment methods for severe corneal alkali burn repair. In this paper, rats were used as the research object. The limbal circular lamelectomy plus central corneal epithelial curettage was used to establish a rat model of complete lack of limbal stem cells in the right eye. The morphology of the ocular surface was observed after 4 weeks. Carrier culture observation, transplantation is performed when the cells are over 90% of the amniotic membrane, the morphology of the right eye of the rat is observed 12 weeks after transplantation, the corneal structure is observed by HE staining, and the differentiation markers of corneal epithelial cells (CEs) are detected by immunofluorescence The expression of CK3+12. After alkali burn of the cornea of rats, the anterior segment of the eye was observed under a microscope, and the corneal turbidity was scored every day. On the 4th, 8th and 12th days after alkali burning, the stained area of the cornea was measured by methylene blue staining to calculate the rate of corneal epithelial healing. The results of the study showed that the corneal repair of rats with alkali burns after 1-3 amniotic membrane transplantation reached a significant standard. The corneal defect area was repaired less than 30%, the matrix infiltration and KP did not decrease and gradually worsened, and a penetrating keratoplasty was given in time.
Keywords
Amniotic Membrane Transplantation, Corneal Alkali Burn, Limbal Stem Cells, Treatment Plan