Volume 30, Issue 1, 2021
DOI: 10.24205/03276716.2020.2025
The Effects of 3-MA on Neurogenic Bladder after Spinal Cord Injury in Rats
Abstract
To investigate the expression of autophagy in the neurogenic bladder cells after spinal cord injury in rats by the intervention of 3-MA of autophagy inhibitor. randomized, controlled trial. Second Hospital of Shandong University, Jinan, China. 27 Wistar rats were randomly divided into 3 groups: sham-operated group, control group, and the 3-
Methyladenine (3-MA) injection group. Spinal cord injury was induced, using a modified
Allen’s method at T10-T11 level. Sham-operated group (Blank group) were subjected to
laminectomy without SCI, while the control group and 3-MA group underwent
laminectomy with SCI. Rats in 3-MA group were injected with 3-MA (2ml/kg body weight
per day), while rats in blank and control group were injected with the same amount of
saline per day. Outcome Measures: The Basso, Beattie, and Bresnahan (BBB) score,
residual urine volume (RUV) and urinary bladder function score (UBFS) were estimated
after 1, 4 and 14 days of surgery. Western blot, immunofluorescence staining and RT-PCR
were used to measure the expression of microtubule associated protein 1 light chain 3
(LC3) and P62 in bladder tissue. Lower BBB score and UBFS, and higher RUV were found
in the 3-MA groups compared to the control group (P < 0.05). In addition, at 1, 4 and 14-
day post-surgery significantly lower LC3 expression was detected in the 3-MA group
compared with the control group (P<0.05), while the P62 mRNA level was significantly
increased at 4- and 14-days post-operation in the 3-MA group compared to control group
(P<0.05); no significant differences in P62 mRNA levels were found between groups at day 1. 3-MA inhibits the expression of autophagy and prevents the recovery of bladder function and motor function of lower extremity
Keywords
3-MA, Autophagy, Neurogenic Bladder, Spinal Cord Injury