Volume 30, Issue 1, 2021
DOI: 10.24205/03276716.2020.2068
Investigation into the effects of IL-10 on the expression of indoleamine 2,3-dioxygenase and SOCS3 in chorionic villi and decidua during early stage of pregnancy
Abstract
The present study aimed to evaluate the expression of indoleamine 2,3-dioxygenase (IDO) and cytokine signaling 3 (SOCS3) in chorionic villi and the decidua after interleukin-10 (IL-10) in early pregnancy. IDO and SOCS3 were expressed in chorionic villi and decidua. IL-10 at 0.1, 1 and 10 ng/ml concentration-dependently decreased the IDO expression in chorionic villi and the decidua tissues; while decreased the expression of SOSC3 in chorionic villi and the decidua tissues. IL-10 at 100 ng/ml transiently suppressed the IDO expression in chorionic villi and the decidua tissues; but had no effect on the SOCS3 expression in the chorionic villi and the decidua tissues. In addition, IL-10 at 100 ng/ml had effect on the IDO and SOCS3 expression at in the chorionic villi and the decidua tissues. IDO expression levels was negatively correlated with SOCS3 levels in chorionic villi and the decidua tissues. IDO expression was the weakest and SOCS3 expression was strongest at 12 h after treating with 10 ng/ml IL-10 in chorionic villi and decidua tissues. Our results indicated IL-10 may down-regulate IDO expression by up-regulating SOCS3 in chorionic villi and decidua tissues, and IL-10 may help prevent allogeneic fetal rejection.
Keywords
IL-10; Indoleamine 2, 3-dioxygenase; suppressor of cytokine signaling 3; maternal–fetal tolerance; chorionic villi; decidua