Volume 30, Issue 1, 2021
DOI: 10.24205/03276716.2020.2069
Preventive effects of sodium butyrate in combination with probiotics on rats with severe burn-induced intestinal injury
Abstract
AIM: We aimed to evaluate the preventive effects of sodium butyrate in combination with probiotics on rats with severe burn-induced intestinal injury.
MATERIALS AND METHODS: Ninety-five male Wistar rats were divided into normal control (n=15), model (n=20), sodium butyrate (n=20), probiotics (n=20) and sodium butyrate + probiotics (n=20) groups. The model of severe scald was established by using boiling water. All rats were administered with corresponding drugs once a day for consecutive 7 days. On the 7th day after modeling, all groups were anesthetized, and their intestinal tissues and blood samples were collected. Intestinal injuries were observed by HE staining. Intestinal cell apoptosis was detected with TUNEL assay. The mRNA expression levels of Bax, EFG, eNOS, iNOS, PPAR?, IL-6, TNF-?, SICAM-1 and SVCAM-1 in intestinal tissues were measured by qRT-PCR, and the protein expression levels of Bax, EGF, NF-?B p65, NF-?B p52, NF-?B p100, ERK, IKKB, eNOS, iNOS and PPAR? were detected by Western blot.
RESULTS: Combining sodium butyrate with probiotics well repaired intestinal damage and restored intestinal barrier function. Western blot and qRT-PCR exhibited that the combination inhibited the expression of BAX but promoted that of EGF in intestinal tissues, thereby alleviating intestinal injury and activating intestinal repair. The combination significantly reduced the intestinal expression of iNOS, whereas elevated that of PPAR?.
CONCLUSIONS: The combination of sodium butyrate with probiotics alleviated intestinal inflammation, inhibited cell apoptosis, improved intestinal repair and protected the barrier function after burn injury. Their combination elevated the expression level of PPAR? but decreased that of NF-?B, which mitigated intestinal inflammation, probably being the important mechanism underlying the protective effects on intestinal tissues.
Keywords
burn, sodium butyrate, probiotics, intestinal inflammation