Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.835
Serum 25 hydroxyvitamin D, Bone Alkaline Phosphatase, Bone Mineral Content and Bone Mineral Density as Important Biochemical Markers for Diagnosis of Nutritional Rickets
Abstract
Purpose:
To investigate the effectiveness of serum 25 hydroxyvitamin D [25 --(OH)D],
bone alkaline phosphatase (BAP), bone mineral content (BMC) and bone mineral density
(BMD) in diagnosis of nutritional
Methods:
Three week old male Sprague Dawley rats (n = 36) weighing 45 55 g (mean
weight = 50 ± 5 g) were randomly assigned to two groups (18 rats/group): control and
rickets groups. Rickets group rats were kept in a dark room and fed vitamin D deficient feed for 35 days. Serum levels of calcium (Ca 2+2+) and phosphorus (P) and activity of alkaline phosphatase (ALP) were assayed using automated biochemical analyzer. Serum
levels of 25 --(OH)D and BAP activity were determined using their respective enzyme
linked immunosorbent assay (ELISA) kits. Bone densitometer was used to assay BMC and
BMD.
Results:
Serum P and 25 --(OH) D levels, and bone mineral densities of proximal, middle and distal femur were significantly lower in rickets group than in control group, but the corresponding activities of ALP and BAP were increased significantly in rickets group,
relative to the control group ( p < 0.05). However, there was no significant difference in
serum Ca 2+ level between the two groups ( p > 0.05). Similarly, the levels of Ca 2+2+, P and
Mg 2+ in the femurs of ricket rats w ere significantly lower than those of control group ( p <
0.05).
Conclusion: These results indicate that serum 25 --(OH)D, BAP, BMC and BMD are important biochemical markers for diagnosis of nutritional rickets.
Keywords
Bone alkaline phosphatase, Bone mine ral content, Bone mineral density, 25 hydroxyvitamin D, Rickets.