Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4024
Association between Has-circ-0067997 with PI3K/AKT Signaling Pathway in Gastric Carcinoma Oncological Characteristics
Abstract
Objective: To investigate the relationship between has-circ-0067997 and PI3K/AKT signaling pathway effecting gastric carcinoma cell line SGC-7901 oncological characteristics.
Methods: Circ-RNA chips used for mining different gene expression, mining different gene, High-throughput sequencing combined with computational systems mining relevant signaling pathway and biological process, Pubmed online database retrieved for miRNA connection with circ-RNA, Crisper/Cas9 structured circ-RNA-knock down cell line, CCK-8 used for cell proliferation analysis, Western blotting and Real-Time PCR used for tested the levels of PI3k/AKT protein and gene expressed.
Results: Circ-RNA chips showed has-circ-0067997 high expressed in chemical drug resisted patients, targeted miRNA High-throughput sequencing indicted has-circ-0067997 related to PI3k/AKT signaling pathway, Circ-RNA interaction online tool mined has-circ-0067997 maybe connect with hsa-miR-127-5p, the biological process and signaling pathway refers to pathway hsa05200: pathways in cancer. The cells’ proliferation increased combined with has-circ-0067997 and hsa-miR-127-5p gene silence. WB and PCR examined PI3k/AKT protein and gene down-regulated combined with has-circ-0067997 and hsa-miR-127-5p gene silence.
Conclusion: has-circ-0067997 connects with hsa-miR-127-5p through PI3K/AKT signaling pathway promotes gastric carcinoma cell line’s oncological characteristics
Keywords
Has-circ-0067997, PI3K/AKT Signaling Pathway, Gastric Carcinoma, Oncological Characteristics