Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4043
Influence of sodium-glucose co-transporter type 2 inhibitor on cardiomyocytes after acute myocardial infarction
Abstract
Objective: To determine the influence of sodium-glucose co-transporter type 2 (SGLT2) inhibitor on cardiomyocytes after acute myocardial infarction (AMI).
Methods: Altogether 36 clean C57B/L6 mice (8-10 weeks old) were chosen and assigned to a control group (con group; Sham operation group), research group (res group; AMI+SGLT2 group), and model group (mod group; AMI group) (each n=12) via the sortition randomization means. AMI models were established by ligating the left coronary artery of each mice in the research and model groups, and each mice in the res group was given 30 mg/kg SGLT2 each day for 2 weeks. Afterwards, the cardiac function indexes, myocardial injury markers, inflammatory factors, oxidative stress, cardiomyocyte apoptosis and associated proteins of mice in the three groups were evaluated and recorded.
Results: Compared with the mod group, the res group showed notable smaller myocardial infarction area. In contrast to the con group, the mod group presented a notable increase in left ventricular end-systolic diameter (LVESD), left ventricular end-diastolic dimension (LVEDD), and myocardial injury markers and a notable decrease in left ventricular ejection fraction (LVEF) and short axis shortening rate (FS). The cardiac function and myocardial injury of the res group were greatly ameliorated after administration with SGLT2. Additionally, in contrast to the con group, the mod group showed a notable increase in the levels of serum TNF-?, IL-1?, and IL-6, and SGLT2 relieved the inflammatory state in the res group. In contrast to the con group, the mod group presented an increase in serum MDA and a decrease in serum SOD, and SGLT2 alleviated the oxidative stress in the res group. Moreover, in contrast to the con group, the mod group presented notably increased apoptosis rate of cardiomyocytes, and SGLT2 reduced the apoptosis rate and improved apoptosis-related proteins in the res group.
Conclusion: For mice with AMI, SGLT2 inhibitor can relieve their myocardial injury and reduce their inflammatory level and oxidative stress in vivo and cardiomyocyte apoptosis.
Keywords
SGLT2 inhibitor, acute myocardial infarction, cardiomyocytes, influence