Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4065
The role and mechanism of miR-210 in unexplained recurrent abortion
Abstract
Purpose: The cause of unexplained recurrent spontaneous abortion (URSA) is unknown. miR-210 regulates vascular endothelial cell proliferation and migration. However, whether it plays a role in URSA remains poorly understood. Our study intends to assess miR-210’s role in URSA.
Materials and Method: Patients with recurrent abortion who underwent uterine surgery were selected as URSA group and normal early pregnancy patients who required termination of pregnancy were selected as a control group. The miR-210 level was detected by real time PCR. HTR-8/SVneo cells were assigned into NC group, miR-210 mimics group, and miR-210 inhibitor group, followed by analysis of cell proliferation by MTT assay, Caspase 3 activity, TNF-? and IL-6 secretion by ELISA, Bax/Bcl-2 expression by Real time PCR and PI3K/Akt signaling protein level by western blot.
Results: miR-210 was significantly lower in URSA group than control group (P <0.05). miR-210 mimics significantly up-regulated miR-210, promoted cell proliferation, decreased Caspase 3 activity and Bax level, upregulated Bcl-2, activated PI3K/Akt, and reduced TNF-? and IL-6 secretion (P <0.05). However, miR-210 inhibitor significantly down-regulated miR-210, inhibited cell proliferation, upregulated Caspase 3 activity and Bax, downregulated Bcl-2, inhibited PI3K/Akt, elevated TNF-? and IL-6 secretion (P <0.05).
Conclusions: miR-210 expression is recued in URSA. Targeting miR-210 can affect cell proliferation and apoptosis, and inhibit inflammatory factors secretion.
Keywords
Unexplained recurrent abortion; miR-210; trophoblast; PI3K / Akt signaling pathway.