Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4075
RELATIONSHIP BETWEEN APOPTOSIS-RELATED PROTEIN BL-X AND CAPASE-3 AND LMO2 GENES IN PROSTATE CANCER
Abstract
Our study intends to investigate the expressional profile of Bcl-xl proteins, Caspase-3 protein expression in different prostate tissues, along with tissue expression of LMO2 gene. A total of 25 cancer tissue samples were collected from surgical removal of prostate cancer from January 2015 to January 2017, along with collection of cancer adjacent tissues and benign prostate hyperplasia tissues. Immunohistochemistry staining was used to measure expression of Bcl-xl and Caspase-3. LMO2 gene expression was quantified by real-time fluorescent PCR. Bcl-xl showed 88% positive expression in cancer tissues, which was significantly higher than cancer adjacent tissues (32%) and benign prostate hyperplasia (45%). Caspase-3 protein presented 16% positive rate in cancer tissues and significantly lower than cancer adjacent tissues (72%) and benign prostate hyperplasia (60%). Bcl-xl presented higher expression whilst caspase-3 had lower expression in cancer tissues, compared to those in benign hyperplasia or cancer adjacent tissues (p<0.05). RT-qPCR showed similar LMO2 mRNA level in cancer adjacent tissues (1.038-fold) compared to benign hyperplasia group (p>0.05), whilst cancer tissues had 897.317-fold higher LMO2 mRNA level (p<0.001). In conclusion, abnormal expression of bcl-xl and caspase-3 proteins are closely correlated with the prostate cancer. LMO2 gene plays a crucial role in the progression of prostate cancer.
Keywords
Prostate cancer, Bcl-xl protein, Caspase-3 protein, LMO2 gene