Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4077
Experimental study of realgar on tumor suppression in lymphoma model
Abstract
Objective: To assess the tumor-suppressive effect of external application of realgar ointment on human lymphoma Raji cell transplantation mouse model.
Methods: Human lymphoma Raji cells were sub cultured and inoculated at dose of 1 x 107 cells to establish lymphoma xenografts mice model which was then assigned into group A (treated with 40% realgar ointment), B (20%), C (10%) and D (model group) followed by analysis of tumor volume and survival at 30,60 and 90 days.
Results: After treatment, tumor volume in group A, B and C was significantly decreased with increased tumor inhibition rates compared to group D. Meanwhile, the initial onset time of tumor suppressor in treatment group was significantly shortened (P< 0.05). The survival rate of mice in A group was significantly lower than that in the other three groups (P<0.0001), while the other three groups had no statistical difference (P>0.05). The concentrations of arsenic in group B (2658.86±1728.38 ug/L) were significantly higher than that in other groups (678.37±285.26 ug/L for group A, 522.55±285.29 ug/L for group C, 34.18±9.39 ug/L for group D) (P< 0.05). There were no significant differences regarding the level of alanine aminotransferase and creatinine value among four groups.
Conclusions: Realgar ointment has obvious anti-tumor effect on mouse model of human lymphoma transplanted tumor in a dose dependent manner, suggesting that it might be a novel approach for treating lymphoma.
Keywords
Realgar, As2S2, Chinese medicine, Lymphoma