Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4082
Discrepant microbiome configurations between hereditary nonpolyposis and sporadic colorectal cancer
Abstract
Objective: Hereditary nonpolyposis colorectal cancer (HNPCC) is a kind of well-known hereditary colorectal cancer (HCRC). This study aims to distinguish the gut microbiome between patients suffering from HNPCC and sporadic colorectal cancer (SCRC).
Methods: 10 HNPCC patients, 14 SCRC, and 14 normal controls were enrolled in this study. 16S rDNA V4 gene sequencing was performed on stool microbiome of these subjects using Illumina Miseq to analyze the fecal microbioata.
Results: The fecal microbiota configurations of patients with HNPCC and SCRC were significantly differed compared to controls (p < 0.002, p = 0.031, respectively), while the variation in SCRC was higher than that in HNPCC. In addition, fecal microbiota of patients with HNPCC and SCRC were significantly different (p = 0.001). The genus Parvimonas was significantly predominant in SCRC patients compared to HNPCC patients and a significant correlation was seen with Fusobacterium, Brevibacillus, Methylobacterium, Granulicella, and Phenylobacterium.
Conclusions: The significant difference in the gut microbiota between HCRC and SCRC highlights the underlying difference in microbial basis of these two phenotypically similar colorectal cancer types. Parvimonas with a higher abundance in SCRC patients relative to those with HCRC, may participate in SCRC pathogenesis and development.
Keywords
Colorectal cancer, gut microbiota, hereditary nonpolyposis colorectal cancer, Parvimonas