Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4086
The Protective Mechanism of Hydroxysafflor Yellow A on the Destruction of Blood-brain Barrier Induced by ?-amyloid
Abstract
Objective: The paper firstly investigated the expression and function of LINC00094, MOV10 and SNHG1/SCAMP1 in human brain microvascular endothelial cells incubated with A?. The paper explored the effect of Hydroxysafflor yellow A on the destruction of the blood-brain barrier induced by ?-amyloid and the regulatory effect on the permeability of the blood-brain barrier in Alzheimer's disease, aiming to provide a new strategy for the treatment of Alzheimer's disease from the perspective of the blood-brain barrier.
Methods: An in vitro BBB model was constructed. qRT-PCR method was applied to detect the expressions of LINC00094, miR-224-5p, and Endophilin-1. Western blot was employed to detect the expressions of Endophilin-1, ZO-1, occludin, and claudin-5. TEER experiment was conducted to test the integrity of BBB. HRP exudation experiment was performed to test BBB permeability. Immunofluorescence was applied to test the expression and distribution of tight junction related proteins.
Results: Hydroxysafflor yellow A can reduce the expression of LINC00094 in ECs incubated with A?, the TEER value significantly increased, the amount of HRP exudation decreased, and the expression of related proteins ZO-1, occludin and claudin-5 were all increased, and reduced BBB permeability in the AD microenvironment.
Conclusion: Hydroxysafflor yellow A combined with LINC00094 knockdown can reduce BBB permeability, and regulate BBB permeability in AD microenvironment through the LINC00094/miR-224-5p/Endophilin-1 pathway.
Keywords
Hydroxysafflor yellow A, blood-brain barrier; LINC00094/miR-224-5p/Endophilin-1; Alzheimer's disease