Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4097
Metformin protects septic rats from myocardial injury
Abstract
This study assessed metformin’s effect on myocardial injury in sepsis rats. Forty male Wistar rats were randomly allocated into sham group, sham/M group (metformin, 100mg/kg), CLP group (cecal ligation and puncture) and CLP/M group (metformin treatment before operation) followed by analysis of ALT?AST?CRE, and plasma TNF-?, IL-1? and cTnI levels by ELISA at 12 and 24 hours after operation, cardiomyocytes apoptosis by TUNEL staining and caspase-3 level by western blot. The levels of ALT, AST, CRE, TNF-?, IL-1? and cTnI showed no differences between sham group and sham/M group after operation. However, they were significantly increased in CLP group and CLP/M group and decreased in CLP/M group compared to CLP group. Myocardial apoptosis indexes in CLP group and CLP/M group were significantly increased than sham group with a lower apoptosis index in CLP/M group than CLP group. At 24 hours after operation, caspase-3 level was significantly higher in CLP group and CLP/M group compared to sham group with a lower level in CLP/M group than CLP group. In conclusion, metformin exerts protective effect on CLP model rats with myocardial injury possibly through reducing myocardial cell apoptosis and inhibiting TNF-? secretion.
Keywords
Metformin; Sepsis; Myocardial injury; Apoptosis