Volume 30, Issue 2, 2021
DOI: 10.24205/03276716.2020.4100
Promising prognostic value of Cadherin-related family member 2(CDHR2) in human cervical cancer
Abstract
Background: In 2018, the incidence (6.6%) and mortality (7.5%) of cervical cancer remained at a relatively high level. The National Cancer Institute and the National Human Genome Institute jointly initiated the Tumor Genome Atlas (TCGA) project, using large-scale sequencing-based genome analysis technology to explore changes in various cancer-promoting and tumor suppressor genes in the development of cancer. Accordingly, it may be possible to screen new biomarkers that reliably detect the prognosis of cervical cancer using this database.
Methods: The corresponding survival data and expression profiling of cervical cancer patients in the TCGA database were compared, and differentially expressed genes (DEG) between cervical cancer and para-carcinoma tissue were identified using a volcano diagram and Venn analysis. Survival analysis verification was performed in the database, and the selected candidate gene was analyzed with Gene Ontology annotation in order to study its functions. Fluorescent quantification PCR was then used to determine the gene expression in fresh cervical cancer tissues and adjacent tissues, after which its correlation with clinical pathological variables were ascertained. Western blot was used for the screening of experimental cells. The biological roles of CDHR2 in the metastatic activities of cervix carcinoma were investigated in vitro through the Cell migration test and Transwell assays
Results: Cadherin-associated family member 2 (CDHR2) was selected as a candidate factor closely associated with the prognostic results of CC patients via the TCGA database. The outcomes of Gene Ontology study revealed that CDHR2 participated in numerous activities biologically, like cell agglutination and differentiative activities of cells. The experimental results confirmed that CDHR2 is upregulated in cervix carcinoma samples, with their clinical stages, tissue and lymph node metastasis being closely related. CDHR2 was also found to be positively correlated with poor clinical prognosis. Additionally, CDHR2 can promote the migrative and invasive activities of oncocytes.
Conclusion: there is an upregulation of CDHR2 in cervix carcinoma, and its increased expression might be an underlying biological marker in predicting inferior prognostic results in cervix carcinoma.
Keywords
cervical cancer, CDHR2, biomarker, prognosis