Volume 30, Issue 3, 2021
DOI: DOI: 10.24205/03276716.2021.5003
Salidroside improves skeletal muscle dysfunction in COPD rats by down regulating SIRT1 signaling pathway
Abstract
Objective: To investigate the effect of salidroside at different doses on skeletal muscle function and SIRT1\ MuRF-1 \ Atrogin-1 expression in COPD rats .
Methods: Forty adult SD rats were divided into 5 groups with 8 rats in each group: blank group (C), COPD model + placebo group (CS), COPD model + low-dose salidroside group (CS + L), COPD model + medium-dose salidroside group (CS + M), COPD model + high-dose salidroside group (CS + H). Each group was taken to be a simple passive smoking hair model smoking rats, the blank group was established to take pseudo-exposed control group model. Group C and CS were given a placebo (normal saline) for 1 month. The CS + L group , CS + M group , and CS + H group were given low dose (5mg / 100g), medium dose (25mg / 100g), High-dose (125mg / 100g) salidroside was administered by gavage for 1 month. Record changes in body weight, use the exhaustive swimming test to evaluate exercise capacity, collect serum, lung tissue, quadriceps, weigh skeletal muscle, and determine the type I fiber ratio of skeletal muscle samples, SIRT1, P-SIRT1, MuRF-1, Atrogin-1 expression level.
Results: (1) Weight comparison: The weight of rats in the C group was higher than that in the CS group, and the statistical difference was considered significant at p<0.05. The weights of the CS + L group, CS + M group, and CS + H group were compared with the CS group and there was an increase in body weight. The statistical differences between the values in each group were considered significant at P <0.05.
(2) Comparison of exhaustive swimming time: Rats in the exhaustive swimming group were longer in the C group than in the CS group, and the statistical difference was considered significant at P <0.05; compared with the CS group in the CS + L group, CS + M group, and CS + H group, Exhaustive swimming time was prolonged, and the statistical differences between the values in each group were considered significant (P <0.05) .(3) Comparison of quadriceps femoris weights: Rat quadriceps femoris weights in the CS group were reduced compared to the C group, and the statistical difference was considered significant at p<0.05; the CS + L group , CS + M group, CS + H group and Compared with the CS group, the quadriceps muscle weight increased, and the statistical differences between the values in each group were considered significant at P <0.05. (4) Type I muscle fiber ratio comparison: Rat type I muscle fiber ratio CS group compared with C group decreased statistically significant (P <0.05); CS + L group, CS + M group, CS + H group and CS group By comparison, the proportion of type I muscle fibers increased, and the statistical differences between the values in each group were considered significant at P <0.05. (5) rats SIRTl , P-SIRTl , MuRF-. 1 , of Atrogin. 1- protein comparison :
the CS group and C compare SIRTl, increased P-SIRT, MuRF-1 expression levels, statisical differences were considered to be significant at p < 0.05; compared with the CS group, the expression levels of SIRT1, P-SIRT, MuRF-1, and Atrogin-1 in the CS + L group, CS + M group, and CS + H group decreased, and the effect level was positively correlated with the intervention dose The statistical differences were considered to be significant at p < 0.05).
Conclusion: Salidroside can improve the skeletal muscle function of COPD model rats by reducing the expression of SIRT1 \ MuRF-1 \ Atrogin-1 .
Keywords
COPD; skeletal muscle dysfunction; SIRT 1 ; salidroside