Volume 30, Issue 3, 2021
DOI: 10.24205/03276716.2021.5006
Hydrogen sulfide reduces skeletal muscle atrophy in pulmonary hypertension rats through Notch signaling pathway
Abstract
Background: To explore whether hydrogen sulfide can atrophy the skeletal muscles of rats with hypoxic pulmonary hypertension, and to explore the preliminary mechanism of action.
Methods: 60 SD rats, male, were divided in a random manner into control group (C), hypoxia group (Sa), hydrogen sulfide group (S), hydrogen sulfide + hypoxia group (S/Sa). The pure passive hypoxic stimulation method was used twice a day, and the hypoxic treatment was continued for 4 months. The control group did not intervene. Intervention with hydrogen sulfide after 3 months of experiment, the intervention ended.After that, the rats in each group were weighed. A small animal ventilator was used to detect lung function, while samples of whole blood, lung tissue, and skeletal muscle were collected for enzyme-linked immunoassay (ELISA) to detect IL-6, IL-8, IL-10, and ROS levels. RT-PCR detects the mRNA content of Notch-1 and Notch-3 in skeletal muscle; Western blot detects the protein content of Notch-1 and Notch-3 in skeletal muscle.
Results: Hydrogen sulfide can cause skeletal muscle dysfunction in hypoxic pulmonary artery rats through oxidative stress-inflammatory mechanisms. Hydrogen sulfide can reduce the level of ROS in lung tissue, serum and skeletal muscle of hypoxic pulmonary hypertension rats and inhibit the expression of IL-6, IL-8, IL-10, and improve hypoxia through suppressed oxidative stress-inflammatory mechanisms The functional status of skeletal muscle in rats with pulmonary hypertension.
Conclusion: Hydrogen sulfide promotes skeletal muscle atrophy in rats with hypoxic pulmonary hypertension through Notch signaling pathway.
Keywords
hypoxia; pulmonary hypertension; hydrogen sulfide; Notch