Volume 29, Issue 4, 2020
DOI: 10.24205/03276716.2020.851
MIRNA-223 Reduced Migration Activity and Proliferation of Nasopharynx Carcinoma Cells Through PI3K/AKT Signaling Pathway by Regulation Of IGF-1R
Abstract
The purpose of our study was to assess and confirm the roles of miRNA-223 expression in the prognosis and migration of nasopharynx carcinoma (NPC) cells, and to explore novel downstream targets. Our findings suggested that miRNA-223 expression in NPC tissues was lower compared to the para-carcinoma tissues. Additionally, downregulation of miRNA-223 expression promoted the proliferation and accelerated the apoptosis of 13-9B cells in a dose-dependent manner. Besides, downregulation of miRNA-223 expression reduced caspase-9 and caspase-3 expression levels, and downregulated Bax expression levels in 13-9B cells. Importantly, expressions of p-Akt1 (phosphorylation-Akt1) and PI3K in 13-9B cells were enhanced by downregulated miRNA-223 expression through activating IGF-1R (insulin-like growth factor 1). Moreover, the influence of miRNA-223 in the growth of 13-9B cells was reversed by activation of IGF-1R. In conclusion, miRNA-223 plays a role as a novel tumor suppressor in the development of NPC through PI3K/Akt signaling pathway by regulation of IGF-1R.
Keywords
miRNA-223, IGF-1R, p-Akt1, nasopharyngeal carcinoma