Volume 29, Issue 3
DOI: 10.24205/03276716.2020.948
Effects of Mir-96 On Proliferation, Apoptosis and Epithelial-Mesenchymal Transition of Oral Tongue Squamous Carcinoma Cells
Abstract
Objective: This study was designed to figure out the effects of miR-96 on proliferation, apoptosis and epithelial-mesenchymal transition (EMT) of oral tongue squamous cell carcinoma (TSCC) cells.
Methods: The expression levels of miR-96 and EMT related proteins were detected through qRT-PCR and WB, and apoptosis was tested through flow cytometry. miR-96 inhibitor vector was established to transfect TSCC cell lines, and TSCC cell proliferation, migration, invasion, apoptosis and EMT were observed. Results: miR-96 was dramatically down-regulated in TSCC cells, and down-regulating it inhibited proliferation, EMT, migration, invasion and apoptosis of cells.
Conclusion: miR-96 is down-regulated in TSCC cells and down-regulating it can inhibit their biological ability, so it may become a new target for TSCC treatment.
Keywords
miR-96, tongue squamous cell carcinoma, proliferation, apoptosis, epithelial-mesenchymal transition