Volume 29, Issue 3
DOI: 10.24205/03276716.2020.953
Comprehensive analysis of the expression pattern of peripheral blood mononuclear cells in rheumatoid arthritis before and after anesthesia
Abstract
Rheumatoid arthritis (RA) is a chronic systemic disease was characterized by inflammatory synovitis. The common causes may be related to heredity, infection and sex hormones. However, the potential effect of rheumatoid arthritis anesthesia on the expression pattern of peripheral blood mononuclear cells has no idea. Therefore, in this study, we explored the expression pattern of peripheral blood mononuclear cells in rheumatoid arthritis patients before and after anesthesia with a variety of analytical strategies. First, download the relevant data in the GEO database, and analyze the differences between normal and disease samples to get the pertinent differences of microRNAs. Then, retrospective targeting of these differentially expressed microRNAs was carried out to obtain the related differentially expressed genes. Then, the protein interaction network of these differential genes is analyzed and the related functional modules are constructed. Finally, a hypergeometric test was carried out to calculate the potential regulatory effects of multiple factors on the module, and a series of ncRNA and TF was identified. 2571 differentially expressed genes were obtained and clustered into 6 functional expression disorder modules. These modules have a tendency to regulate biological processes such as regulation of protein ubiquitousness, regulation of neuron apoptosis process. At the same time, they are mainly involved in PI3K-Akt signaling pathway, FoxO signaling pathway and JAK-STAT signaling pathway. In addition, in view of pivot analysis, 76 ncRNAs and 4 TFs were found to drive dysfunctional expression modules in rheumatoid arthritis. Based on the results of this study, we can provide a new way for biologists and pharmacists to study rheumatoid arthritis, and provide valuable reference for follow-up treatment programs.
Keywords
rheumatoid arthritis, anesthesia, dysfunctional expression module, peripheral blood mononuclear cells, expression pattern