Volume 29, Issue 3
DOI: 10.24205/03276716.2020.707
Down-regulation of CK17 Inhibits Cell Proliferation and EMT in Gastric Cancer Cells
Abstract
Cytokeratin 17 (CK17) is an epithelial basal cell / muscle keratin released by the activated keratinocytes. It is not present in most somatic cells, but in some stem cells and some malignant tumors in cervical cancer, ovarian cancer, head, and neck tumors, etc. A systematic research on the control and function of CK17 in gastric cancer is not performed recently. The study explored CK17 regulatory mechanism for the spread, apoptosis, resettlement, incursion of gastric cancer cells. RT-PCR, Western blot detected mRNA and CK17 protein concentrations in 72 gastric cancer nerves and 5 gastric cancer cells. A link in the middle of CK17 representation and clinical pathological parameters and prognosis had been studied within diseased person’s patients with gastric cancer. Lentivirus has been leading down-regulation of CK17 in gastric cancer cells. CCK-8 detected cell spreading assay and Tran’s well detected plate cloning assay. Cell migration and invasiveness. Manifestation of EMT-connected proteins within gastric cancer cells was calculated using Western blot. CK17 expression within gastric cancer nerves and cell areas was substantially increased, its level of representation closely linked to stage TNM, lymph node metastases within patients with gastric cancer. Prognostic studies found the diseased persons having minimal manifestation of CK17 possessed a longer period of subsistence concerning gastric cancer. Cell experimental findings showed the downregulation of CK17 prevented the spread, resettlement, penetration about gastric cancer cells. Levels of expression of N-cadherin and Vimentin proteins have been substantially reduced, however appearance of E-cadherin and Δ-catenin has increased significantly. CK17 is highly expressed within gastric cancer and is thoroughly associated with phase TNM, lymph connection metastases and prognosis. CK17 can promote propagation, resettlement, incursion, EMT of gastric cancer cells and inhibit apoptosis of cells.
Keywords
gastric cancer; CK17; cell proliferation; EMT.