Volume 29, Issue 3
DOI: 10.24205/03276716.2020.920
Predictive Value of Mir-155 and Mir-203 in Children with Chronic Gastritis
Abstract
This study inquired into the predictive value of miR-155 and miR-203 in children with chronic gastritis. In this study, 86 children with chronic gastritis treated in our hospital from February 2015 to May 2017 were collected as the observation group (OG), and another 73 healthy children who underwent physical examination in our hospital during the same period were collected as the control group (CG). QRT-PCR was employed to detect the profile of related indexes in serum of the two groups. ROC curve was applied to analyze the diagnostic value of miR-155 and miR-203 in children with chronic gastritis and Helicobacter pylori (Hp). Multivariate Logistic regression was adopted to analyze the risk factors of Hp infection in children with chronic gastritis. Pearson test was used to analyze the correlation between serum miR-155 and miR-203 and inflammatory indexes IL-6 and TNF-α in the OG. The miR-155 was remarkably higher while the miR-203 was lower in the OG than in the CG. According to the ROC curve, the area under the curve (AUC) of miR-155, miR-203 and their combination in diagnosing chronic gastritis in children was 0.789, 0.703 and 0.833 respectively, and their AUCs in diagnosing Hp infection in children was 0.776, 0.775 and 0.812 respectively. Family history of digestive tract diseases, IL-6, family size, miR-155 and miR-203 were independent risk factors for Hp infection in children with chronic gastritis in children. A negative correlation was identified between miR-155 and miR-203, between miR-203 and IL-6 expression, and between miR-203 and TNF-α expression, while a positive correlation was found between miR-155 and IL-6 expression and between miR-155 and TNF-α expression. In conclusion, miR-155, miR-203, miR-155 and miR-203 may be potential diagnostic indicators of chronic gastritis and Hp infection in children.
Keywords
miR-155; miR-203; chronic gastritis in children; Helicobacter pylori