Volume 29, Issue 3
DOI: 10.24205/03276716.2020.924
Exploring the Biomarkers of Clinical Diagnosis by Analyzing the Tissue Specificity and Commonality of Echinococcosis Based on The Pathogenic Modules in Echinococcosis
Abstract
Hydatid disease caused by echinococcus multilocularis is a latent zoonotic parasitic disease. It is not easy to be discovered and diagnosed, but it can seriously threaten the health of the patient and even lead to death. Therefore, exploring its key biomarkers and its pathogenic mechanism are essential. Based on disease-related co-expression modules, we showed the relationships between pivot regulators and echinococcosis, including transcription factors (SMADC7, EP300 and TRP53, etc.) and miRNAs (miR-1323p, miR-384-5p and miR-20b-5p, etc.). Functional enrichment analysis showed that they can regulate a series of symptoms and complications of echinococcosis by mediating functions and pathways such as antimicrobial humoral immune response mediated by antimicrobial peptide, regulation of MAP kinase activity and acute inflammatory response. In addition, we validated the reliability of results through the label-free differential protein analysis. We found that most of the disease-causing genes were tissue-specific, while the five genes APOA1, F2, SERPING1, APOH, and CLU have tissue commonality, which identified as key biomarkers for clinical diagnosis. In general, our work not only clarifies the tissue specificity and commonality of echinococcosis, but also clarifies the key biomarker for clinical diagnosis, which providing medical scientists with a solid theoretical foundation for more in-depth research.
Keywords
echinococcosis, pathogenic modules, tissue specificity and commonality, biomarkers host, and then move to the appropriate tissue for development. This process can cause a series of